Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities.
AUTOR(ES)
Katiyar, S S
RESUMO
The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393566Documentos Relacionados
- NADPH-binding protein of the neutrophil superoxide-generating oxidase of guinea pigs.
- The cytosolic subunit p67phox contains an NADPH-binding site that participates in catalysis by the leukocyte NADPH oxidase.
- Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities.
- Origins of metabolic diversity: substitution of homologous sequences into genes for enzymes with different catalytic activities.
- Putative mediators of insulin action: regulation of pyruvate dehydrogenase and adenylate cyclase activities.