Anatomopathology of bovine pericardium implants preserved in differents concentrations of glutaraldehyde in mice abdominal wall. / Anatomopatologia de implantes de pericárdio bovino conservados em diferentes concentrações de glutaraldeído em parede abdominal de camundongos

AUTOR(ES)

Cláudia Borges da Costa

DATA DE PUBLICAÇÃO

2009

RESUMO

This experiment aimed at recognizing and quantifying the possible macro and microscopic tissue alterations that may occur with the application of bovine pericardium preserved in different concentrations of glutaraldehyde on the abdominal wall. Besides, it also intended to identify the possible interferences in the implant incorporation, according to the variation in the concentration of each solution. Fresh bovine pericardium, after cleaning and the removal of fat, were fractioned and treated with 98% glycerin solution for 30 days (control groups) or with 0.625%, 1% and 1.5% glutaraldehyde solution for 18 days (experimental groups). The fragments were implanted on the abdominal wall of 60 mice (n=15 per treatment) for 7, 14 and 30 days. After each of these periods, five animals of each group were sacrificed for collection, and macro and microscopic analysis of tissue samples. It was observed that the inflammatory reaction in the groups treated with glycerin and 0.625% glutaraldehyde was similar in terms of inflammatory cells infiltration and the repairing process. The groups whose implants were treated with 1% glutaraldehyde chronically presented (30 days) not only more intense fibrosis and necrosis, when compared to other glutaraldehyde groups, but also tissue destruction. These were also observed in the animals that received the pericardium treated with 1.5% glutaraldehyde, analyzed after 14 and 30 days, what leads to the conclusion that this concentration is unviable for the treatment of biological membranes.

ASSUNTO(S)

reação inflamatória pericárdio bovino medicina veterinaria inflammatory reaction glutaraldehyde bovine pericardium implante. implant. glutaraldeído

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