Analytical studies and antibacterial essential oil extracted from leaves of Pimenta dioica Lindl / Estudo analítico e antibacteriano do óleo essencial extraído das folhas da espécie Pimenta dioica lindl

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The vegetal specie Dioecious spice Lindl known as Jamaicas spice is a 6 to 10 meters high tree that belongs to Mirtaceae family and has the origin in Central America and Indias west. In Brazil, the plant is found in big scale in Bahias state. Its leaf contains a essential oil of big economic value in the international market, due to the high content of eugenol (its majority compound) that is widely used in the chemistry and pharmaceutical industry. In this work, was promoted the extraction of essential oil of the leafs of the Dioecious spice Lindl by the hydrodistillation method, using a Clevengers system. Was observed that the maximum volume of oil extracted is extracted in a time of 3,5 hours with a 2,45% m/m yield. Besides the physic constants of density, refraction rate, solubility, color and appearance, was possible by UVs spectrophotometric techniques, gas chromatography and spectrometric of mass identify with security the eugenol as majority constituent of the essential oil of the Dioecious spice Lindl. Through the extreme pattern method and addition pattern, using gas chromatography and UVs spectrophotometric, respectively, wasnt possible quantify the eugenol in the essential oil, that presented content of 74,1  1,4%. In the apply of essential oil of Dioecious spice Lindl species and of the pattern of eugenol as bactericidal agents, was used the Bauer-Kirby Method to Bacillus cereus, Serratia marcescens, Listeria monocytogenes, Enterococcus faecalis, Salmonella thyphi and Chromobacterium violaceum bacteria, and the antibiotics Penicillin, Ampicillin, Cefotaxime, Vancomycin, Cefoxitin, Erythromycin, Gentamicin, Tetracycline and Novobiocin, for the comparison. Can be realized that in this research, the essential oil and the pattern of eugenol were more efficient than the antibiotics tested for the microorganisms Listeria monocytogenese and Enterococcus faecali, whilst the pattern of eugenol showed less efficient than the oil. For Bacillus cereus, Serratia marcescens and Salmonella thyphi the oil only wasnt more efficient than the Tetracycline, Erythromycin, Cefoxitin, cefotaxime, Penicillin and Ampicillin antibiotics.

ASSUNTO(S)

pimenta dioica lindl atividade antibacteriana Óleo essencial quimica dos produtos naturais bactericidal activity essential oil dioecious spice lindl eugenol

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