Analytical method development for determination of codeine in human plasma by on-line SPE-HPLC-MS/MS / Desenvolvimento de metodologia analitica para determinação de codeina em plasma humano utilizando extração solido-liquido "on-line" acoplado ao HPLC-MS/MS

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using on-line solid-phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. Method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing high-throughput for codeine determination. SPE on-line along monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng/mL. Method showed an excellent intra-assay and inter-assay precision ranged from 2.33 to 7.25% (CV%) as well as great intra-assay and inter-assay accuracy, ranging from -2.35 to 9.97% (RE%). SPE-LC-MS/MS method provided selectivity, robustness, accuracy, precision and high-throughput to assess codeine pharmacokinetics in human plasma samples

ASSUNTO(S)

codeina extração em fase solida cromatografia liquida de alta eficiencia codeine high performance liquid cromatography solid-phase extraction espectometria de massa

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