Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination.
AUTOR(ES)
Sochard, M R
RESUMO
The passive hemagglutination assay was explored as a sensitive test of immunological reactivity of endotoxin and other antigens prepared from selected strains of Vibrio parahaemolyticus. The tannic acid procedure for passive hemagglutination, commonly used with protein antigens, was the only procedure yielding good results with V. parahaemolyticus protein extracts, endotoxins, and related preparations. These results were probably due to the presence of large amounts of protein in the V. parahaemolyticus endotoxins as determined by earlier work referenced in the text. Glucose and galactose as possible antigenic determinants in the endotoxin of a Vibrio strain were tested by inhibition tests. Cross-reactions were observed between endotoxin preparations, but were low in hemagglutination, suggesting reactions of common generic antigens. The ability of V. parahaemolyticus endotoxins to stimulate production of antibodies was determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273289Documentos Relacionados
- Strain differentiation of Neisseria gonorrhoeae by reverse passive hemagglutination.
- Detection of rotavirus antibody by inhibition of reverse passive hemagglutination.
- Quantitative assay of diphtherial toxin and of immunologically cross-reacting proteins by reversed passive hemagglutination.
- Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.
- Hemagglutination and adhesiveness of epidemiologically distinct strains of Vibrio parahaemolyticus.
