Analysis of Urs(g)-Mediated Glucose Repression of the Gal1 Promoter of Saccharomyces Cerevisiae
AUTOR(ES)
Flick, J. S.
RESUMO
Repression of GAL1 expression during growth on glucose is mediated in part by cis-acting promoter elements designated URS(G). We show that oligonucleotides containing sequences from two regions of URS(G) confer glucose repression upon a heterologous promoter. Repression caused by URS(G) is dependent on trans-acting factors of the glucose repression pathway and is independent of orientation or location within a promoter, suggesting that URS(G) contains binding sites for a glucose-activated repressor protein(s). Genetic analysis identified three apparently novel genes (URR1, URR3 and URR4) that are specifically required for URS(G)-mediated repression and may encode such repressor proteins. Mutations in the URR genes suppress the defect in URS(G) derepression caused by a snf1 mutation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1204850Documentos Relacionados
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