Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.

AUTOR(ES)

Guerrant, G O

RESUMO

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.

Documentos Relacionados

• Identification of mycobacteria by high-performance liquid chromatography.
• Analysis of short-chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column.
• Rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.
• Rapid determination of serum 5-fluorocytosine levels by high-performance liquid chromatography.
• High-performance liquid chromatography patterns of Mycobacterium gordonae mycolic acids.