Analysis of repeatability in spotted cDNA microarrays
AUTOR(ES)
Jenssen, Tor-Kristian
FONTE
Oxford University Press
RESUMO
We report a strategy for analysis of data quality in cDNA microarrays based on the repeatability of repeatedly spotted clones. We describe how repeatability can be used to control data quality by developing adaptive filtering criteria for microarray data containing clones spotted in multiple spots. We have applied the method on five publicly available cDNA microarray data sets and one previously unpublished data set from our own laboratory. The results demonstrate the feasibility of the approach as a foundation for data filtering, and indicate a high degree of variation in data quality, both across the data sets and between arrays within data sets.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=135761Documentos Relacionados
- Discovery and analysis of inflammatory disease-related genes using cDNA microarrays
- Normalization strategies for cDNA microarrays
- Optimizing spotting solutions for increased reproducibility of cDNA microarrays
- Identificação de marcadores moleculares para o câncer de tireóide por cDNA microarrays
- Accuracy and calibration of commercial oligonucleotide and custom cDNA microarrays