Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin
AUTOR(ES)
Bracesco, N., Sosa, V., Blanc, L., Contreras, V., Candreva, E.C., Salvo, V.A., Hocart, S., Mechoso, B., Nunes, E.
FONTE
Braz J Med Biol Res
DATA DE PUBLICAÇÃO
16/07/2018
RESUMO
DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip.
Documentos Relacionados
- Avaliação da erva-mate (Ilex paraguariensis) na adipogênese e sinalização da insulina
- Atividade antioxidante do chá mate (Ilex paraguariensis)
- Evaluation of the Redox State of Wistar Rats Submitted to High-Fat Diet Supplemented With Infusion of Ilex paraguariensis
- The anti-obesity potential of Ilex paraguariensis: results from a meta-analysis
- Methylxanthines accumulation in Ilex species: caffeine and theobromine in erva-mate (Ilex paraguariensis) and other Ilex species