Analysis of populational variation and characterization of secondary metabolites in the leaves of Lychnophora granmongolense (Duarte) Semir &Leitão Filho. / Análise de variação populacional e caracterização dos metabólitos secundários presentes nas folhas de Lychnophora granmongolense (Duarte) Semir &Leitão Filho

AUTOR(ES)

Leandro de Santis Ferreira

DATA DE PUBLICAÇÃO

2010

RESUMO

One of the main characteristics of plants of the Asteraceae family is their capacity to produce a huge diversity of secondary metabolites having therapeutic and toxic effects. An analytical method was developed based on HPLCDAD which was used to verify the existence of variations in Lychnophora granmongolense (Duarte) Semir &Leitão Filho secondary metabolite levels among species within populations and between distinct populations and to characterize the major secondary metabolites. Through this method three compounds, namely vicenin-2, quercetin and pinocembrin were identified by co-injection of standards. This identification was based on the comparison of retention time with isolated standards and the UV spectrum. Four sesquiterpenes lactones centratherin, 4,5-dihydrocentratherin, lycnopholide and 15-deoxygoyazensolide were identified by mass spectrometry techniques coupled with HPLC (HPLC-DAD-MS e HPLC-DAD-MS/MS). In the populational variation study, the results show large qualitative and quantitative differences among species within populations and between populations for high and medium polarity secondary metabolites. Eight triterpenes and eight acetyl triterpenes were identified by apolar compound analysis. However, small, insignificant variation was observed for these particular metabolites. Thus, this study contributed to an increase in the knowledge of the metabolic rhythm of this specie.

ASSUNTO(S)

flavonóides. técnicas hifenadas variation in populations hyphenated techniques lychnophora granmongolense flavonoids. lactonas sesquiterpênicas variação populacional secondary metabolites lychnophora granmongolense sesquiterpenes lactones metabólitos secundários

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