Analysis of Parvovirus mRNA by Sedimentation and Electrophoresis in Aqueous and Nonaqueous Solution

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RESUMO

Adenovirus-associated virus (AAV)-specific RNA present in the cytoplasm of cells coinfected with a helper adenovirus was analyzed by sucrose gradient sedimentation and gel electrophoresis. In aqueous conditions both gels or gradients revealed three AAV RNA components corresponding to 30S, 27S, and 20S and having apparent molecular weights of 2.6 × 106, 1.75 × 106 to 1.8 × 106, and 0.9 × 106 to 1.0 × 106, respectively. In nonaqueous, denaturing solvents only the 20S AAV RNA species was observed. For this reason, and because they would be apparently significantly larger than a single AAV DNA strand, both the 30S and 27S species are believed to result from conformational or aggregation effects in the aqueous nondenaturing systems. It is concluded that only a single RNA molecule having a molecular weight of approximately 0.9 × 106 to 1.0 × 106 is synthesized by AAV.

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