Analysis of in vitro binding of U1-A protein mutants to U1 snRNA.

AUTOR(ES)

Boelens, W

RESUMO

Despite the great sequence similarity between U1A and U2B", both proteins do have a difference in RNA binding specificity and in the way they bind to their cognate RNAs. The U1A protein is able to bind in vitro U1 RNA independently of other factors. The U2B" protein binds specifically to U2 RNA in the presence of the U2A' protein only. We have compared the effect on RNA binding of multiple double point mutations at analogous positions in the U1A and U2B" protein. The results obtained show that amino acids at almost all of the analogous positions tested in U1A and U2B" have a comparable qualitative effect on RNA binding although the quantitative effect of mutations on U2B" is more severe than on U1A. Using U1A mutants with internal duplications a distinct area of the RNP motif of the U1A protein was identified which appears not to be directly involved in U1 RNA binding. In addition, roles of the highly conserved RNP1 and RNP2 sequences of the N-terminal RNP motif of the U1A protein, are investigated by replacing them with the analogous U1-70K sequences.

Documentos Relacionados

• In vitro synthesis of vertebrate U1 snRNA.
• Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
• An additional long-range interaction in human U1 snRNA.
• A novel approach to describe a U1 snRNA binding site
• U1-U2 snRNPs interaction induced by an RNA complementary to the 5' end sequence of U1 snRNA.