ANALYSIS OF ANTIMICROBIAL POTENTIAL OF EXTRACT, FRACTIONS AND PURE COMPOUNDS OBTAINED FROM Piper solmsianum C. DC. VAR. solmsianum (PIPERACEAE). / ANÁLISE DO POTENCIAL ANTIMICROBIANO DE EXTRATO, FRAÇÕES E SUBSTÂNCIAS PURAS OBTIDAS DE Piper solmsianum C.D.C. VAR. solmsianum (PIPERACEAE)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The species Piper solmsianum belong to the family Piperaceae, which is comprised of about 700 species, and is widely distributed in the tropical and subtropical zones of world. The chemical of P. solmsianum has not been fully investigated, and there are few studies relating to its bilogical activities. This work evaluated the antimicrobial properties of the crude methanol extract (CME), fractions (hexane, dichloromethane, and ethyl acetate) and compounds (conocarpan, conocarpan methylated, eupomatenoid-3, eupomatenoid-5, and orientin) obtained from P. solmsianum. The minimal inhibitory concentrations (MIC) were determined through the method of diluition in agar, showing excellent activity, particularty against the Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, Staphylococcus saprophyticus and Streptococcus agalactiae. The components extracted of the P. solmsianum also showed activity against fungi, particularly the dermatophytes Epidermophyton floccosum, Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrum. The bioautographic test revealed three inhibition zones, relating to the conocarpan, eupomatenoid-5, and another compound, as yet unidentified. The antimicrobial activity of P. solmsianum appears to be related to the presence of conocarpan, eupomatenoid-5, orientin, and another compound not yet identified. The hystopatologic evaluation of inflammation, induced by Staphylococcus aureus, demonstrated that the groups treated with CME of P. solmsianum showed the process of regeneration of the tissue in lesser time (less delay in the process of lesion repair), when compared with the control group. The toxicity assay using Artemia salina demonstrated that the CME and the fraction hexane of P. solmsianum presents moderate toxicity (LD50 of 89,98 e 87,85 μg/mL, respectively), the fraction ethyl acetate showed weak activity (LD50 of 877, 66 μg/mL), and the fraction dichloromethane and the conocarpan substance showed high toxicity (LD50 of 2,91 and 2,92 μg/mL, respectively). However the compounds conocarpan methylated, eupomatenoid-5, eupomatenoid-3 and orientin did not present toxicity (LD50 >1000 μg/mL).

ASSUNTO(S)

piper solmsianum conocarpano orientin produtos naturais conocarpan eupomatenóide farmacia activity antimicrobial piper solmsianum orientina eupomatenoid atividade antimicrobiana

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