Análises transcricionais no estudo da expressão gênica de Paracoccidioides brasiliensis em condições que mimetizam nichos do hospedeiro

AUTOR(ES)

Alexandre Melo Bailão

DATA DE PUBLICAÇÃO

2008

RESUMO

Paracoccidioides brasiliensis is a fungal human pathogen with a wide distribution in Latin America. It causes paracoccidioidomycosis, the most widespread systemic mycosis in Latin America. The fungus causes infection through host inhalation of airborne propagules of the mycelial phase of the organism. These particles reach the lungs, and disseminate to other organs and tissues. Although gene expression in P. brasiliensis had been studied, little is known about the genome sequences expressed by this species during the infection process. Here we describe the analysis of differentially expressed genes of P. brasiliensis yeast cells obtained from infected animals and during incubation of the fungus with human blood thus mimicking the hematogenic route of the fungal dissemination. Additionally, we also identified the differentially expressed genes of the fungus treated with human plasma, mimicking infection sites with inflammation. The data revealed that genes related to iron acquisition, melanin synthesis and cell defense were specially upregulated in the mouse model of infection. The upregulated transcripts of yeast cells during incubation with human blood were those predominantly related to cell wall remodeling/synthesis. Genes related to fatty acid degradation, protein synthesis, sensing of osmolarity changes, cell wall remodeling and cell defense were upregulated in yeast cells treated with human plasma. The differential expression was confirmed by dot blot, northern blot and sqRT-PCR assays. The generated data can facilitate functional studies of novel regulated genes that may be important for the survival and growth strategies of P. brasiliensis in different host niches.

ASSUNTO(S)

resposta nicho-específica espresão gênica paracoccidioides brasilienses genetica molecular e de microorganismos

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