Análises molecular e morfométrica em populações naturais de Eupemphix nattereri, 1863 (Amphibia: Anura: Leptodactylidae) do Brasil Central

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Genetic diversity is necessary to the adaptation of populations to environmental changes, and could be measured by quantitative and molecular methods. The knowledge of species variability is also fundamental for conservation, and is an important characteristic. To assess species genetic diversity, population genetic structure must be evaluated, which means, the knowledge of heterogeneous distribution of non random alleles and genotypes in space and time, as a result of evolutive process such as mutation, migration, natural selection and genetic drift, that act distinctly in the context of each species and population. To verify the genetic structure of Eupemphix nattereri, molecular analyses were conducted in 11 populations of this leiuperid, sampled in Central Brazil (Goias State, Rio Claro and Mato Grosso do Sul). Such analyses were estimated using dominant RAPD markers. Besides, 11 morphometric characters were evaluated only in populations of Goias State. Genetic and morphometric matrixes were analyzed as a function of geographical origin. Correlation among genetic, geographical, morphometric, micro and macroenviromental were analyzed by the Mantel test. Genetic data indicated high levels of genetic diversity (around 0.30), B according to three different approaches (? ? and ? ), among the eleven populations. ST, P Mantel tests did not reveal a significant positive correlation between genetic variation and geographical distance, indicating that locally geographical populations are not genetically similar, even in distances smaller than 50 km. Discriminant analysis on 11 measurements showed considerable overlap between populations from different geographical areas, but there is a marginally significant correlation (p= 0.08) between genetic and morphometric distances. This correlation is not causal in terms of the relationship between phenotype and genotype, and indicates common spatial structures. Thus, isolation-by-distance processes, despite the low statistical power in the analyses, could explain population divergence in Eupemphix nattereri. So, our results indicated that Eupemphix nattereri populations were not structured in space, showing a random distribution. Besides, genetic data demonstrated lack of isolation-by-distance and lack of gene flow connecting populations.

ASSUNTO(S)

anuro - genética de populações - brasil morfometria population structure eupemphix nattereri genetica fluxo gênico rapd biologia animal eupemphix nattereri rapd gene flow estrutura populacional morphometrics

ACESSO AO ARTIGO

Documentos Relacionados