Análise morfométrica e molecular da alveolite induzida em ratos com diferentes modalidades de tratamento / Molecular and morphometric analysis of induced dry socket in mice with different treatment conditions

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Dry socket is an inflammatory postoperative complication that undertakes sockets of recently extracted teeth. The incidence of such complication varies from 1 to 4% and might reach up to 30%. The objective of this study was to analyze the biological mechanisms involved in the repair process of intentionally infected sockets in mice; compare different treatment conditions and correlate the results of two different analysis (microscopic and molecular). 84 mice were used in this study, divided according the following groups: I: uninfected socket; II: infected socket without any treatment; III: infected socket treated with irrigation of 2% sodium iodide and 3% hydrogen peroxide solution at 1:1 proportion; and IV: infected socket submitted to curettage, physiological saline solution irrigation and fulfillment with metronidazole base paste. The animals were killed at a postoperative period of 6, 15 and 28 days. A quantitative analysis was performed using a RealTimePCR to evaluate the genes expression involved [Collagen Type I (COL-I), vascular endothelial growth factor (VEGF), osteocalcin (OCN), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and tumor necrosis factor-alpha (TNF-alpha)], in the repair process, correlating its expression with the microscopic characteristics observed in both qualitative and quantitative manner. Based in the results of the microscopic and molecular analysis, it can be concluded that the RUNX2, OCN and TNF-alphamarkers can be used as indicators to evaluate the dry socket bone neoformation and inflammatory infiltrate quantity. The ALP and VEGF markers did not represented appropriately what was observed microscopically. Although the dry socket treatment with metronidazole base paste promotes an increase in the bone neoformation density at 28 days, no difference was found among the treatments.

ASSUNTO(S)

osteocalcin runx2 fosfatase alcalina hydrogen peroxide metronidazole alvéolo seco collagen type i colágeno tipo i peróxido de hidrogênio vascular endothelial growth factor cirurgia bucal fator de necrose tumoral alfa alkaline phosphatase oral surgery osteocalcina runt-related transcription factor 2 dry socket metronidazol. iodeto de sódio sodium iodide tumor necrosis factor-alpha fator de crescimento do endotélio vascular

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