Analise microbiologica de canais radiculares e radiografica de dentes de cães com lesão periapical induzida

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The value of dogs in endodontic research is well known by the regularity of their use. Many investigations are conducted in order to observe periapical response and repair on the periapical tissues of this experimental model. Microorganisms are the main etiological agents in pulpal and periapical disorders, therefore comprehension of the dog root canal microflora is important. The objective of this study was to analyse microbiological canal roots and radiograph induced periapical lesions in dog s teeth. Twenty six lower premolars of five mongrel dogs were used. Their chambers were accessed and their pulps removed and left open to the oral environment for the total period of 120 days. Clínical and radiographic controls were used. The radiographs were evaluated by Imagelab 2.4 software to measure the area and perimeter images of the induced periapical lesions. Samples from the root canais were collected after 120 days. Methodology for handling, culture and incubation for growth of strict anaerobe species was used. A total of 70 cultivable isolates were recovered from 34 different species, rallging from 0 to 9 per canal. Facultative anaerobe species comprised 77.14% of the samples and 22.8% were strict anaerobic species with one microbiota predominantly Gram-positive. The most frequent genera recovered from the canais were: Streptococcus, Staphy/ococcus, Neisseria, Propionibacterium, Actinomyces and Prevotella. Measurements of the radiographic image data of the lesions were 0.06 cm2 for the second premolars, 0.12 cm2 for the third premolars and 0.17 cm2for the fourth premolars. In conclusion, the root canal microbiota of dog teeth with induced periapical lesion, by exposure to the oral environment, is predominantly composed by facultative anaerobes and Gram-positive bacteria, represented by Streptococcus and Staphy/ococcus genera. The results suggest a positive relationship between the tooth length and size of the periapical lesion.

ASSUNTO(S)

endodontia cão como animal de laboratorio bacterias

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