Analise histoquimica, ultra-estrutural e morfometrica do efeito de drogas anti-inflamatorias não esteroides (naproxeno e indometacina) sob a regeneração da nadadeira caudal de teleosteo, Cyprinus carpio (carpa) / Histochemical, ultra-structural and morphometric analysis of the effect of nonsteroidal anti-inflammatory drugs (naproxen and indomethacin) under the tail fin regeneration of teleostm Cyprinus carpio (carp)

Petra Karla Bockelmann

The fins of teleosts, when partially amputated, they pass for a quick regenerative process called epimorphic regeneration, characterized by the formation of a mass of undifferentiated cells, by the differentiation of these cells, by the synthesis and the deposition of the extracellular matrix and morphological restoration. The regeneration of the fin is extremely sensitive to external physical and chemical factors such as temperature variations, light intensity, the action of some environmental contaminants and the action of some drugs that can interfere in the regenerative capacity of teleost fins. There are some studies that show that nonsteroids anti-inflammatory drugs can interfere somehow in the tissue restoration of many organisms, as they inhibit the action of ciclooxygenase enzyme and, consequently, the conversion of arachidonic acid in prostaglandins, elements that execute important roles in cell protection, growth, angiogenesis and in the production of extracellular matrix. In this sense, this study aimed to evaluate the effects of nonsteroid anti-inflammatory drugs, naproxen and indomethacin, during the regenerative process of the teleost tail fin Cyprinus carpio (carp). Therefore, experiments were undertaken in five groups: the control group fish, group of fish in touch with naproxen in doses of 15.6 mg/L, and three groups of fish in contact with indomethacin in doses of 10, 20 and 30 mg/L each. The fish were anesthetized and their fins transversally amputated and, after amputations the fish were divided among the five groups described above and were left in the aquaria until the occurrence of regeneration. The animals were anesthetized, sacrificed and the regenerating fins excised and fixed in intervals of 1, 2, 4, 5, 6, 8, 10 and 12 days after amputation. The samples were processed in order to permit a histochemical, ultra-structural and morphometric analysis of the possible alterations in the regenerative process of the tail fin of the teleosts in contact with the drugs mentioned above. The group treated with naproxen and indomethacin in a 10 mg/L dose showed a regenerative process similar to the control group, thus, it did not affect the formation of the epidermal layer, the formation of blastema, and the differentiation of blastemal cells, as well as its synthesis, deposition, organization and mineralization of the lepidotrichial matrix and the synthesis of actinotrichia during the process of regeneration of the tail fin. However, the fish treated with indomethacin in doses of 20 and 30 mg/L presented a delay in the regenerative process of the lepidotrichia and actinotrichia when compared to the control group fish. Detailed studies about the mechanisms of nonsteroids anti-inflammatory drugs action and the action of these drugs under the expression or inhibition of expression of some genes involved in the teleost tail fin regenerative process could explain more precisely the reason of the differences of effect between these two drugs

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