Análise do perfil de similaridade da microbiota subgengival de indivíduos com periodontite

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The objective of this study was to analyze possible differences in the profile of similarity of the subgingival microbiota in subjects with clinical diagnosis of periodontal disease. Two hundred and twenty subjects with periodontal disease, participant of different clinical research on periodontal therapy developed for the group of research of the Periodontology Graduate Program of the Guarulhos University (UnG) were included in this study. The clinical parameters evaluated at 6 sites per tooth at baseline were: probing depth (PD), clinical attachment level (CAL), visible plaque, gingival bleeding, bleeding on probing and suppuration. Nine subgingival biofilm samples were collected per subject, 3 samples in each of the following initial PD categories: shallow (PS _ 3mm), moderate (PS 4-6 mm), and deep (PS _ 7mm). The samples were evaluated for the presence and levels of 40 bacterial subgingival species using ckeckerboard DNA-DNA hybridization. The samples were evaluated for the levels and proportions of 40 bacterial subgingival species using checkerboard DNA-DNA hybridization. The microorganism of the red and orange complex presented the higher mean level of counts and proportion. With the objective to study the profile of similarity of the individuals, dendogram had been constructed in accordance with the microbiological data. Nine clusters were formed at >45% similarity. The levels of 19 species had been significantly different between the groups. The counts of species of the orange and red complexes were lowest in groups III, IV and VIII, with the inferior levels at 5 x 105 bacteria. The more elevated ratios of the red complex had been observed in groups V (44.0%) and VII (42.6%), followed of the groups II (39.6%), VI (34.5%) and I (27.6%), and of the groups IX (24.9%), IV (22.7%), VIII (20.7) and III (12.2%). About orange complex, the groups I (39.6%) and VII (38.9%) showed the more elevated proportions. On the other hand, unexpectedly, in some groups the benefit microorganisms were in high ratios. The blue complex represented 17.5% in group III and 26% in group IV; and the yellow complex represented 20.6% in group III and 16.2% in group VIII. In conclusion, there are differences in the profile of similarity of subgingival microbiota of the individuals with clinical diagnosis of periodontal disease. Other microorganisms can to do a role in the periodontal disease etiology, above the classic pathogens of the red complex .

ASSUNTO(S)

sondas de dna dna probe microbiota subgengival doença periodontal subgingival microbiota periodontal disease odontologia

ACESSO AO ARTIGO

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