Análise das células inflamatórias e da proteína anexina 1 no modelo de artrite experimental e reumatóide humana. / Analysis of the inflammatory cells and annexin 1 protein in the experimental model arthritis and in the human rheumatoid .

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Rheumatoid arthritis (RA) is a disease characterized by auto-antibody formation, chronic inflammation and joint oedema resulting in injury of affected cartilage and adjacent bone. In the treatment of this disease glucocorticoids (GC) have been used, wich induce genic expression of anti-inflammatory mediators, such as the annexin 1 protein (Anx-A1), and proinflammatory ones. In the pathogenesis of arthritis inflammatory cells such as mast cells are involved. Under activation these release potent proinflammatory mediators, wich are stored in their citoplasmic granules. In the present study we have analyzed the occurrence of mast cells during collageninduced arthritis (CIA) and their effect on neutrophils, a kind of leukocyte that promotes joint inflammation. Paw volume analysis of experimental mice showed that clinical signs of rheumatoid arthritis gradually increased until 42 days after collagen immunization. Knee and digit analysis showed neutrophil influx into the joints on the 21st and 32nd days, respectively, after CIA. Histologically, knees and digits presented different levels of leukocyte infiltration, showing that these joints are affected at different stages of the inflammation. Neutrophil migration was accompanied by an augmented number of mast cells in the digits and an intense process of degranulation in the knees, on the 21st day after CIA. To evaluate the effects of these inflammatory cells in CIA the animals were treated with prednisolona, commonly used in clinical treatment, and nedocromil, a membrane mast cell stabilizer drug. After drug administration, although the occurrence of arthritis was not modified, neutrophilic migration to the tissue was reduced. The prednisolona GC produced a marked mast cell migration to the joints and nedocromil action inhibited neutrophil proliferation and migration, with a profound effect on the digit joints. Collagen immunization induced an inflammatory process mainly characterized by mast cell activation and degranulation, with a profound effect on the maintenance of the inflammatory process and neutrophil recruitment. From this point of view, the profile of the cells studied in the digits and knees suggested that the joints are affected in a time-dependent manner during CIA development. In the studies related to human rheumatoid arthritis (RA) we investigated endogenous mobilization of the Anx-A1 protein and its formyl peptide receptor like 1 (FPRL-1) in the circulating lymphocytes of active and remissive patients. Ultrastructural analysis showed co-localization and high expression of Anx-A1 and FPRL-1, especially in the nucleus and cytosol of the lymphocytes of active RA patients in relation to remissive ones. These data suggest that endogenous Anx-A1 is activated during RA and this may be correlated to FPRL- 1. In this way we may conclude that the study of cellular types involved in arthritis and that the role that Anx-A1 protein may play during disease development may contribute to the development of new anti-inflammatory therapies based on the Anx- A1 system.

ASSUNTO(S)

1. anexina 1. 2. artrite experimental e reumatóide humana. 3. mastócito. 4. neutrófilo. 5. linfócito. morfologia

ACESSO AO ARTIGO

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