Análise das alterações microscópicas em glândulas salivares menores e fígado após o transplante alogênico de células tronco hematopoéticas / Analysis of microscopic changes in minor salivary glands and liver after allogeneic hematopoietic stem cell transplantation

AUTOR(ES)

Tânia Cristina Benetti Soares

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

29/03/2012

RESUMO

Chronic graft-versus-host disease (cGVHD) is an alloimmune inflammatory process, which results from a donor-origin cellular response (T cells) against host tissues. The organs mostly affected are skin, oral mucosa, salivary glands and liver. The aim of this study was to evaluate the histopathological findings of minor salivary glands (MSG) and liver of patients affected by cGVHD, comparing the findings each other and with those of the patients who did not develop cGVHD after HSCT and patients who were not underwent HSCT. MSG and liver samples from patients who underwent myeloablative HLA-matched HSCT from sibling donors, between 1994 and 2006, were analyzed. Fifty-seven patients were selected, being 36 with oral and hepatic cGVHD and 21 without GVHD. The diagnosis of cGVHD was defined through clinical/ laboratory criteria and follow-up. Samples from non-transplanted patients were also evaluated (19 MSG and 20 liver specimens). The specimens were stained with haematoxylin &eosin, periodic acid-Schiff (PAS), Masson s trichrome, reticulin and Perls, and immunolabeled for CD45, CD45RO, CD68, CD4, CD8, CD138, and AE1/AE3. The criteria defined in the classification proposed by the consensus meeting of the National Institutes of Health were used for the evaluation of all samples of both organs. Digital images of the PAS stained sections were used to estimate the MSG acinar area. In the liver of patients who developed GVHD it was observed a statistically significant increase in the number of CD45RO and CD8 immunostained cells, comparatively with the other 2 groups...Note: The complete abstract is available with the full electronic document.

ASSUNTO(S)

doença enxerto-hospedeiro imunohistoquímica graft vs host disease immunohistochemistry

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