Análise da variabilidade genética e Avaliação da fixação biológica de nitrogênio entre acessos de amendoim (Arachis hypogaea L.) / Analisys of genetic variability and assessment of biological nitrogen fixation among peanut (Arachis hypogaea L.) accessions

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The peanut is a crop worldwide cultivated and it presents a high potential to biological nitrogen fixation (BNF). However peanut is considered a very promiscuous crop, what makes necessary a hard work of selection of rhizobial efficient and competitive strains to produce inoculants for this crop. The selection of an efficient association depends of the knowledge of genetic variability between both symbionts, since the levels of compatibility may reveal specificity association levels. The knowledge of more efficient and specific associations may optimize of BNF by selection of symbiont partners. The aim of this study was evaluate, by a molecular marker (RAPD), the genetic variability among 29 accessions of A. hypogaea L. and evaluate the BNF of clusters representing accessions, above non inoculated conditions. Were tested 55 arbitrary primers and selected 31, that generated 145 amplified fragments, which 35 showed polymorphism (24%), with averages of 4.67 and 1.13 fragments and polymorphic fragments by primer, respectively. In despite of the substantial diversity among peanut genotypes by several morphologic characteristics, low variation had been detected using DNA based markers. In the dendrograms constructed using the RAPD data was possible to observe two principal clusters among the accessions studied. It was not observed clustering neither by the origin of accessions nor about characterization of subspecies. The BNF capability differences of nine accessions in non inoculation conditions were evaluated in two soils. The evaluations of this experiment permitted conclude that the accessions IAC Tatu-ST, IAC 886 Runner, Sapucaia vermelha, Sapucaia Bege and CV Tatuí had shown a higher performance when compared with the other accessions. It was also observed that, the definition of the accessions more efficient to BNF, necessarily is not related to its genetic relationship, once this accessions belonged to distinct groups by assay RAPD.

ASSUNTO(S)

diversidade genética amendoim genetic diversity fbn agronomia ap-pcr

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