Análise da expressão gênica de macrófagos murinos infectados por fungos patogênicos (Paracoccidioides brasiliensis e Histoplasma capsulatum)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Paracoccidioides brasiliensis and Histoplasma capsulatum are the thermo-dimorphic fungi that cause the Paracoccidioidomycosis and Histoplasmose, respectively. Paracoccidioides brasiliensis causes a human systemic mycosis with high incidence in Latin America and Histoplasma capsulatum is specially found in the United States. Little is known about the global regulation of genes involved in the innate immune host response to this fungus. In the presente work was investigated the kinetic profile of peritoneal macrophage infected with P. brasiliensis and Histoplasma capsulatum. Total RNA from infected and non infected macrophages was extracted, hybridized onto arrays and analyzed. The results showed in this work to suggest which the pathogens have been induced in macrophages a dynamic gene modulation that can create favorable conditions for fungal persistence. By the other hand the macrophages to induced genes related with fungicide activity, probably in a tentative of elimination of the fungus. In addition, considering the simultaneous analyses of differential gene expression for the P. brasiliensis reported before by our group, at six hours post infection, was proposed a model at molecular level for the P. brasiliensis-macrophage early interaction. In this regard, the induction of gene expression by P. brasiliensis specially related which its adaptation in macrophage microenvironment of macrophage probably is a strategy developed by this fungus for its viability into the host cells.

ASSUNTO(S)

histoplasma capsulatum biologia molecular paracoccidioides brasiliensis

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