Análise da atividade linfoproliferativa de células esplênicas murinas frente a extrato de seis plantas medicinais da flora catarinense / Analysis of the citotoxic and lymphoproliferative activity of methanolic exctracts of medicinal plants on murine spleen cells

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Some compounds have been used as immunomodulatory agents, enabling the function of the immune system to be modified, stimulating or suppressing it. There has been increasing interest in the study of therapeutic activities of plant extracts in recent years, including their immunomodulatory activity. The aim of the present work is to identify and evaluate the action of extracts of the medicinal plants Calophyllum brasiliensis, Ipomoea pes-caprae, Matayba elaegnoides, Maytenus robust, Rubus imperialis and Vernonia scorpioides on the development of spleen cells from mice using the in vitro cellular proliferation assay. The assay was carried out in 96-well microplates using Eagle Dulbeccos Modified Medium (DMEM) supplemented with fetal bovine serum, L-glutamin, HEPES and sodium pyruvate. The murine spleen cells were obtained by mechanical rupture of the spleen (5x103 cells/well). The methanol extracts were used at 1, 5, 10 and 20 mg/well, while the phytohemagglutinin (PHA) and pokeweed mitogen (PWM) were both used at 0.5 mg/well. The cell proliferation was performed by incubating the cellular suspension with the plant extract, isolated or simultaneously with PHA and PWM. The negative proliferation control consisted of cells and DMEM, while the positive controls consisted of cells and PHA and PWM in isolation. The cell culture was kept at 37 oC with 5% of CO2 for 72 hours, and the cell proliferation was quantified by means of the blue tetrazolium reduction assay (MTT assay). The results were expressed as percentage growth, and were analyzed by the Kruskal-Wallis and Mann-Whitney tests. The Calophyllum brasiliensis extract showed dosedependent induction of the cell proliferation, with a significant increase in cell proliferation when incubated in isolation and together with the PHA (p<0.029), increasing the PHA effect and suggesting stimulation of the T lymphocytes. The Ipomoea pes-caprae extract induced dose-dependent cell proliferation, with a significant increase in proliferation when incubated in isolation and together with PWM (p<0.043), indicating, with other data, that T and B lymphocytes are activated. The Matayba elaegnoides extract showed dose-dependent induction of cell proliferation with significant induction of growth, both in isolation and with PWM (p<0.030), suggesting that the extract has a stimulatory effect on T and B lymphocyte activation and proliferation. The Maytenus robust extract showed no interference on cell proliferation when incubated in isolation and simultaneously with PWM (p>0.083), but showed a suppressant response when incubated simultaneously with PHA. The diversity of the results obtained did not allow us to make any inferences concerning the activity of this extract. The Rubus imperialis and Vernonia scorpioides extracts presented inhibition of the cellular proliferation both when incubated in isolation and simultaneously with PWM (p<0.043). As a result, this work presents plants with immunomodulatory activities that deserve to be highlighted and studied in further depth, due to their stimulatory effect, e.g. Calophyllum brasiliensis and Ipomoea pes-caprae, as well as their inhibitory effect, Rubus imperialis and Vernonia scorpioides

ASSUNTO(S)

matayba elaegnoides matayba elaegnoides plantas medicinais maytenus robusta vernonia scorpioides resposta imune imunomodulação rubus imperialis maytenus robusta rubus imperialis immune response calophyllum brasiliensis farmacia ipomoea pes-caprae immunomodulation vernonia scorpioides calophyllum brasiliensis ipomoea pescaprae

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