Análise da atividade antimicrobiana dos óleos de dopaíba (C opaifera officinalis ) e da melaleuca ( Melaleuca alternifolia ) sobre Fusobacterium nucleatum e Por phyromonas gingivalis: determinação das concentrações inibitórias e bactericidas mínimas e efeito de concentrações subinibitórias sobre a agregação / Antimicrobial activity of copaiba (Copaifera officinalis) and melaleuca (Melaleuca alternifolia) oils on Porphyromonas gingivalis and Fusobacterium nucleatum: determination of minimum inhibitory and bactericidal concentrations and subinibitory effect on the aggregation

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

18/08/2011

RESUMO

The oral cavity is a complex microbial habitat that has more than 500 bacterial species as components of the microbiota. Periodontal health is established when there is equilibrium between pathogens and host. The chlorhexidine digluconate is one of the most commonly used oral antibiotics, however, this substance has been associated with some undesirable side effects. Copaiba and melaleuca oils have been studied as important herbal medicines because of their effects, including antibacterial action. Based on the principle that the copaiba oil and tea tree have an antimicrobial activity and that is no sufficient data in the literature using these herbal medicines against Porphyromonas gingivalis and Fusobacterium nucleatum, Minimum Inhibitory Concentration (MIC) and minimum bactericidal concentration (MBC) tests of Fusobacterium nucleatum (ATCC 25586) and Porphyromonas gingivalis (ATCC 3327) related to chlorhexidine digluconate and oils coming from Copaifera officinalis and Melaleuca alternifolia, were prepared. Assays were performed to determine the subinibitory concentration and the capacity of those bacteria to autoaggregation and coaggregation when exposed to subinibitory concentrations, previously tested. Medium and medium added Tween 80 were used as a control. All oils used had their composition analyzed by gas chromatography-mass spectrometry. The tea tree oil mainly chemical compounds were identified as terpin-4-ol, -terpinen, -terpinen, terpinolene and 1,8-cineole while copaiba oil presented as its main constituents trans-caryophyllene, germacrene B, -humulene, germacrene D and -copaene. The MIC results for F.nucleatum were similar to the CBM data in all solutions. For the bacterium P. gingivalis, all solutions tested inhibited bacterial growth, however, the results obtained during the determination of CBM showed that the copaiba oil was bacteriostatic. All solutions tested inhibited the autoaggregation process but only copaiba oil was effective in inhibiting the coaggregation between F.nucleatum and P. gingivalis. These data suggest that all the solutions tested in this study have relevant changes in the normal development of P.gingivalis and F.nucleatum as well in the influence of the autoaggregation process of F.nucleatum yet Copaiba oil also demonstrated to have remarkable properties to change coaggregation between the bacteria used in this study.

ASSUNTO(S)

fitoterapia fusobacterium nucleatum fusobacterium nucleatum Óleos vegetais phytotherapy plants oils porphyromonas gingivalis porphyromonas gingivalis

ACESSO AO ARTIGO

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