AnÃlise cariotÃpica em Lonchorhina aurita e Trachops cirrhosus (Chiroptera: Phyllostomidae) usando diferentes tÃcnicas citogenÃticas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

In this work chromosomal features of the species of Phyllostomidae bats Lonchorhina aurita (Lonchorhininae) and Trachops cirrhosus (Phyllostominae) were studied. Different cytogenetic techniques were used, including conventional analysis, G and C-banding, staining with silver nitrate and base-specific fluorochromes. Data regarding the conventional analysis obtained to L. aurita and T. cirrhosus revealed the karyotype constitution of 2n=32,XY (NF=60) and 2n=30,XY (NF=56), respectively. These results are in agreement with those described in the literature, except for the morphology of the sexual chromosomes, indicating geographical chromosomal variation for those species. Additionally, we suggest that the acrocentric morphology of the X chromosome of T. cirrhosus is an autapomorfic character and possibly was the result of a pericentric inversion. The C-banding revealed pericentromeric CH in all autosomes and in the X. The Y chromosome was almost completely heterochromatic in both species. The staining with AgNO3 revealed NORs located in the short arm of pair 13 in L. aurita and in the long arm of pair 11 in T. cirrhosus. In both species the CMA3/DA/DAPI staining revealed the R-banding pattern with CMA3, while, the fluorochrome DAPI showed an uniform staining in all the chromosomes. The pericentromeric CH regions of some chromosomes presented positively staining with CMA3. The sequential staining AgNO3/CMA3/DAPI revealed that NORs are CMA3 positive in L. aurita and neutral CMA3 in T. cirrhosus, indicating a heterogeneity regarding the composition of NOR-associated CH

ASSUNTO(S)

genetica chiroptera, citogenÃtica, bandeamento cromossÃmico, fluorocromos, ron chiroptera, cytogenetic, chromosome banding, fluorochromes, nor

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