Anaerobic Transport in Escherichia coli Membrane Vesicles*
AUTOR(ES)
Konings, Wilhelmus N.
RESUMO
Anaerobic β-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of α-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic β-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport in vitro. Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=427240Documentos Relacionados
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