An unusual 5' splice sequence is efficiently utilized in vivo.
AUTOR(ES)
Fischer, H D
RESUMO
The minor adult chicken alpha-globin gene (alpha D) has an intron splicing sequence at the 5' end of the second intron that begins with the dinucleotide G-C rather than the usual G-T. To understand what role this splice sequence plays in the processing and maturation of nuclear RNA to cytoplasmic RNA, we have analyzed the intron processing of the alpha D-globin transcript in both heterologous (monkey) and homologous (chicken) cells using simian virus 40 and retrovirus vectors, respectively. In both cell types, both introns of the alpha D-globin gene are efficiently and precisely removed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345144Documentos Relacionados
- The primary structure of the duck alpha D-globin gene: an unusual 5' splice junction sequence.
- Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo.
- The A1 and A1B proteins of heterogeneous nuclear ribonucleoparticles modulate 5' splice site selection in vivo.
- Splicing of a yeast intron containing an unusual 5' junction sequence.
- An intron enhancer containing a 5' splice site sequence in the human calcitonin/calcitonin gene-related peptide gene.