An outbreak of gastroenteritis associated with astrovirus serotype 1 in a day care center, in Rio de Janeiro, Brazil

AUTOR(ES)

Silva, AMV, Leite, EG, Assis, RMS, Majerowicz, S, Leite, JPG

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2001-11

RESUMO

Between June 4th and June 20th1996 rotavirus, adenovirus, and astrovirus (HAstrV) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the Bertha Lutz day care center, in Rio de Janeiro. All fecal samples were analyzed by polyacrylamide gel electrophoresis (PAGE), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme immunoassays (EIA), and electron microscopy (EM). Nine of them (33%) showed positive results for HAstrV by at least one of the employed methodologies. Eight were positive by RT-PCR and EIA, and six by EM. All positive samples were inoculated onto HT-29 (human colon adenocarcinoma) cultured cells for HAstrV isolation and seven were positive after three passages. The sequencing analysis of eight RT-PCR products (449 bp) from gene that codifies VP2 protein, showed a total nucleotide identity among them and 98% with HAstrV-1 (strain Oxford type 1). This is the first report of a gastroenteritis outbreak associated with HAstrv-1 in a day care center in Rio de Janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis.

Documentos Relacionados

• Noroviruses associated with acute gastroenteritis in a children's day care facility in Rio de Janeiro, Brazil
• A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil
• Outbreak of human calicivirus gastroenteritis in a day-care center in Sydney, Australia.
• Molecular detection of human astrovirus in an urban sewage treatment plant in Rio de Janeiro, Brazil
• Characterization and seroepidemiology of a type 5 astrovirus associated with an outbreak of gastroenteritis in Marin County, California.