An in silico pipeline to filter the Toxoplasma gondii proteome for proteins that could traffic to the host cell nucleus and influence host cell epigenetic regulation

AUTOR(ES)

Syn, Genevieve, Blackwell, Jenefer M, Jamieson, Sarra E, Francis, Richard W

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

28/05/2018

RESUMO

Toxoplasma gondii uses epigenetic mechanisms to regulate both endogenous and host cell gene expression. To identify genes with putative epigenetic functions, we developed an in silico pipeline to interrogate the T. gondii proteome of 8313 proteins. Step 1 employs PredictNLS and NucPred to identify genes predicted to target eukaryotic nuclei. Step 2 uses GOLink to identify proteins of epigenetic function based on Gene Ontology terms. This resulted in 611 putative nuclear localised proteins with predicted epigenetic functions. Step 3 filtered for secretory proteins using SignalP, SecretomeP, and experimental data. This identified 57 of the 611 putative epigenetic proteins as likely to be secreted. The pipeline is freely available online, uses open access tools and software with user-friendly Perl scripts to automate and manage the results, and is readily adaptable to undertake any such in silico search for genes contributing to particular functions.

Documentos Relacionados

• Lysosomes of Toxoplasma gondii and Their Possible Relation to the Host-Cell Penetration of Toxoplasma Parasites
• Host Cell Penetration of Toxoplasma gondii
• Toxoplasma gondii Uses Sulfated Proteoglycans for Substrate and Host Cell Attachment
• Attachment of Toxoplasma gondii to host cells is host cell cycle dependent.
• Factors Enhancing the Host-Cell Penetration of Toxoplasma gondii