An evaluation of cerebrospinal fluid oligoclonal banding confirmed by immunofixation on agarose gel.
AUTOR(ES)
George, P M
RESUMO
The cerebrospinal fluid (CSF) from 115 consecutive patients undergoing diagnostic lumbar puncture or myelography was examined to determine the usefulness of immunofixation, following agarose gel electrophoresis, in the detection of oligoclonal IgG. All electrophoretic patterns were evaluated with and without immunofixation, and the interpretation of 9% of specimens was altered by immunofixation. The demonstration of oligoclonal IgG was shown to be more reliable in the diagnosis of multiple sclerosis than other indices of intrathecal synthesis of IgG. It is concluded that immunofixation should be used routinely when examining CSF for oligoclonal banding.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1027438Documentos Relacionados
- A comparison of cellulose acetate immunofixation with polyacrylamide gel electrophoresis for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.
- Oligoclonal banding in the cerebrospinal fluid of patients with postencephalitic Parkinsonism.
- Detection of transcribed amplified DNA sequences by a modification of the method of DNA renaturation in an agarose gel.
- Lymphoma cells in cerebrospinal fluid confirmed by chromosome analysis
- Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.