An ecdysone response element in the Drosophila hsp27 promoter
AUTOR(ES)
Riddihough, Guy
RESUMO
It has previously been shown that a region of ˜100 bp in the Drosophila hsp27 promoter is sufficient to confer ecdysone inducibility on a heterologous gene. We now show, using binding and DNase I footprinting assays, that a 23-bp hyphenated dyad within this sequence forms a protein-binding site, and that this is sufficient for inducibility. The sequence shows partial homology with mammalian steroid receptor binding sites. UV crosslinking identifies an 80- to 90-kd protein that binds specifically to this sequence and is thus a candidate for the ecdysone receptor.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553843Documentos Relacionados
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