An Appraisal on the Source-to-Sink Relationship in Plants: an Application of Desorption Electrospray Ionization Mass Spectrometry Imaging

AUTOR(ES)

Freitas, Julia R. L., Vendramini, Pedro H., Melo, Júlio O. F., Eberlin, Marcos N., Augusti, Rodinei

FONTE

J. Braz. Chem. Soc.

DATA DE PUBLICAÇÃO

2018-01

RESUMO

Amino acids/ureides and carbohydrates are the main nutrients for the plants growth. These metabolites are continuously translocated through the plant organism via well-known transportation mechanisms. The well-established source-to-sink relationship involves the transfer of these nutrients from mature (source) to young (sink) leaves. Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is used herein to determine the spatial distribution of amino acids/ureides and carbohydrates in the imprintings of Mentha × piperita L. leaves at three distinct maturation stages: young, expanding, and mature. Hence, chemical images of some typical carbohydrates (glucose, sucrose, sorbitol/mannitol) and free amino acids/ureides (phenylalanine, leucine, histidine, aspartate, asparagine, tyrosine, glutamate, allantoic acid) clearly point to a source-to-sink relationship, as the young leaves are the final destination for some of these key metabolites. These results therefore reveal the high potential of DESI-MSI to investigate biochemical processes in plants, a still underexplored area.

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