An algorithm for protein engineering: simulations of recursive ensemble mutagenesis.
AUTOR(ES)
Arkin, A P
RESUMO
An algorithm for protein engineering, termed recursive ensemble mutagenesis, has been developed to produce diverse populations of phenotypically related mutants whose members differ in amino acid sequence. This method uses a feedback mechanism to control successive rounds of combinatorial cassette mutagenesis. Starting from partially randomized "wild-type" DNA sequences, a highly parallel search of sequence space for peptides fitting an experimenter's criteria is performed. Each iteration uses information gained from the previous rounds to search the space more efficiently. Simulations of the technique indicate that, under a variety of conditions, the algorithm can rapidly produce a diverse population of proteins fitting specific criteria. In the experimental analog, genetic selection or screening applied during recursive ensemble mutagenesis should force the evolution of an ensemble of mutants to a targeted cluster of related phenotypes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=49801Documentos Relacionados
- Engineering multiple properties of a protein by combinatorial mutagenesis.
- Biomedical Engineering: An International Symposium
- Weighted-ensemble Brownian dynamics simulations for protein association reactions.
- Molecular engineering: An approach to the development of general capabilities for molecular manipulation
- An efficient method for isolation of promoter mutations after oligonucleotide-directed mutagenesis.
