Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

AUTOR(ES)
FONTE

Brazilian Journal of Microbiology

DATA DE PUBLICAÇÃO

01/12/2011

RESUMO

Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.

ASSUNTO(S)

solid state fermentation agro-industrial wastes amylase enzyme optimization stability bacillus sp

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