AMPEROMETRIC SENSOR BASE OF AN IMPRESSION OF POLYMER MOLECULAR PROTOPORPHYRIN IX WITH IRON FOR THE DETERMINATION OF 4-AMINOPHENOL / SENSOR AMPEROMÉTRICO A BASE DE UM POLÍMERO DE IMPRESSÃO MOLECULAR COM PROTOPORFIRINA IX DE FERRO PARA A DETERMINAÇÃO DE 4-AMINOFENOL

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

This work presents an analytical study on determination of 4-aminophenol (4-APh) with a system based on molecurlaly imprinted polymer (MIP) and iron protoporphyrin IX as biomimetic sensor of the peroxidase enzyme. The MIP synthesis was performed by the precipitation method and the analytical determinations by amperometric measurements with a glassy carbon electrode modified with the biomimetic system. The best analytical conditions established for 4-APh determination were: applied potential of -0.1 V vs. Ag/Agl, 0.05 mol L-1 Tris buffer solution, pH 7 and hydrogen peroxide concentration of 100 μmol L-1. Under such optimized conditions, a good linear sensor response was observed in a concentration range from 10 to 90 μmol L-1 (r>0.998), with detection and quantification limits of 3 and 10 μmol L-1, respectively. Experiments performed with tap and river water samples spiked with 4-APh resulted in 93 to 112 % recovery values, suggesting the viability of using the MIP/iron protoporphyrin IX system as biomimetic sensor for determinations of 4-APh in real samples.

ASSUNTO(S)

sensor biomimético biomimetic sensor 4-aminophenol 4-aminofenol iron protoporphyrin ix molecurlaly imprinted polymer protoporfirina de ferro polímero de impressão molecular quimica analitica

ACESSO AO ARTIGO

Documentos Relacionados