Amino acid sequence of the precursor region of MOPC-315 mouse immunoglobulin heavy chain.

AUTOR(ES)

Jilka, R L

RESUMO

Partially purified mRNA coding for the MOPC-315 heavy immunoglobulin alpha chain was translated in a reticulocyte lysate containing 20 labeled amino acids. Radiolabeled MOPC-315 heavy chain precursor protein, purified by preparative gel electrophoresis and immunoprecipitation, was sequenced by Edman degradation. The labeled phenylthiohydantoin amino acid obtained in each cycle was identified and quantitated by high-pressure liquid chromatography. The precursor sequence of 18 amino acids, Met-Lys-Val-Leu-Ser-Leu-Leu-Tyr-Leu-Leu-Thr-Ala-Ile-Pro-His-Ile-Met-Ser, preceded the sequence corresponding to the NH2 terminus of the mature secreted heavy chain.

Documentos Relacionados

• Cell-free translation of immunoglobulin messenger RNA from MOPC-315 plasmacytoma and MOPC-315 NR, a variant synthesizing only light chain.
• Amino acid sequence of a mouse immunoglobulin mu chain.
• Complete amino acid sequence of a mouse immunoglobulin alpha chain (MOPC 511).
• Localization of Idiotypic Antigenic Determinants in the Fv Region of Murine Myeloma Protein MOPC-315
• Amino acid sequence of the first 217 residues of a mouse heavy chain (MOPC 47A) with a domain deletion.