Ambient-temperature primary nonselective enrichment for isolation of Salmonella spp. from an estuarine environment.
AUTOR(ES)
Kaper, J B
RESUMO
A primary, nonselective, ambient-temperature enrichment procedure for isolation of Salmonella spp. is described. The procedure was superior to elevated-temperature selective enrichment for Salmonella when estuarine water samples were examined. Five Chesapeake Bay stations were monitored, over an 8-month period, for the presence of salmonellae. Of 72 water and sediment samples collected, 17 (23.6%) yielded Salmonella spp. Seven serotypes were identified among the isolates. A seasonal pattern was noted for the incidence of the salmonellae. A most probable number procedure, performed by membrane filtration and nonselective enrichment, yielded Salmonella most probable number indices as high as 110 per 100 g of sediment. The results suggest that new methods, such as the one described in this report, are required for isolation of human intestinal pathogens from estuaries and coastal waters.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170775Documentos Relacionados
- Proteus mirabilis fimbriae: identification, isolation, and characterization of a new ambient-temperature fimbria.
- Proteus mirabilis ambient-temperature fimbriae: cloning and nucleotide sequence of the aft gene cluster.
- Delayed secondary enrichment for the isolation of salmonellae from broiler chickens and their environment.
- Broth medium for enrichment of Vibrio fluvialis from the environment.
- Semisolid media for isolation of Salmonella spp. from coastal waters.