Amazon emulsions as cavity cleansers: antibacterial activity, cytotoxicity and changes in human tooth color


Rev. bras. farmacogn.




ABSTRACT The copaiba oleoresin, Copaifera multijuga Hayne, Fabaceae, is a phytotherapeutic agent with antimicrobial activity. This study evaluated the antibacterial activity and cytotoxicity of, and tooth color changes caused by four copaiba oil emulsions (Emulsion 1, 10% CM; Emulsion 2, 10% C. multijuga + 1% biotech product; Emulsion 3, 30% C. multijuga; and Emulsion 4, 30% C. multijuga + 1% biotech product). The antibacterial activities against microorganisms causing dental caries (Streptococcus mutans ATCC25175, S. oralis ATCC10557, S. salivarius ATCC7073, and Lactobacillus casei ATCC7469) were tested using three parameters: minimum inhibitory concentration, minimum bactericidal concentration, and cell viability by fluorescence microscopy. The emulsions were assessed for cytotoxicity by means of the hemolytic assay and cell culture (murine fibroblast cells NHI3T3) using Alamar BlueTM. The dentin color change caused by the emulsions was examined at 10 s, 30 s, and 10 min. The emulsions showed antibacterial activity against the microorganisms tested with an MIC of 125 µl/ml. The minimum bactericidal concentration was higher than minimum inhibitory concentration for the tested microorganism and the fluorescence confirmed that the cells were viable at minimum inhibitory concentration values. The emulsions had a hemolytic activity of 71.16% (Emulsion 3) and 44.67% (Emulsion 4) at a concentration of 30 µl/ml. In cell culture assay, NHI-3T3 cells treated with the emulsions showed 6–16% viability. Emulsion 1 caused clinically imperceptible color change in dentin at 10 s (ΔE = 3.21), Emulsion 2 at 30 s (ΔE = 2.70) and 10 min (ΔE = 3.08), and Emulsion 4 at 10 min (ΔE = 3.03). Emulsion 3 caused color change at all times tested. This research documented positive data regarding antibacterial activity, cytotoxicity, and tooth color changes when using copaiba oleoresin emulsions, showing its potential for use in dentistry.

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