Altered restriction endonuclease cleavage pattern of simian virus 40 DNA.
AUTOR(ES)
Vogel, T
RESUMO
Three different groups of temperature-sensitive mutants of simian virus 40, isolated and characterized by Chou and Martin (J. Virol. 13:1101--1109, 1974), have been analyzed by using restriction endonucleases. Differences between the restriction endonuclease cleavage pattern of these mutants and that of the standard simian virus 40 strain have been mapped. These include the following observations: (i) tsD202 carries a defective HaeIII cleavage site at position 0.9 map units; (ii) tsB204 exhibits a defective HaIII site at position 0.21 and a defective HinIII site at 0.655 map units, and (iii) tsC219 carries a new HinIII site at position 0.15. We have isolated a few wild-type revertants from each of the temperature-sensitive mutant strains; each displays the endonuclease cleavage pattern of its parental temperature-sensitive strain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353091Documentos Relacionados
- Specific Cleavage of Simian Virus 40 DNA by Restriction Endonuclease of Hemophilus Influenzae*
- Altered activity of restriction endonuclease Mn1-I cleavage of mouse satellite DNA.
- A restriction endonuclease cleavage map of mouse mitochondrial DNA.
- Site specificity of bleomycin cleavage in purified and intracellular simian virus 40 DNA.
- A Colinear Map Relating the Simian Virus 40 (SV40) DNA Segments of Six Adenovirus-SV40 Hybrids to the DNA Fragments Produced by Restriction Endonuclease Cleavage of SV40 DNA