Age-related biological activity of South American rattlesnake (Crotalus durissus terrificus) venom
AUTOR(ES)
Furtado, M. F. D., Santos, M. C., Kamiguti, A. S.
FONTE
Journal of Venomous Animals and Toxins including Tropical Diseases
DATA DE PUBLICAÇÃO
2003
RESUMO
An in vitro and in vivo comparative study was performed on the effects of Crotalus durissus terrificus venoms from a mother and its 15 newborns. The venoms were tested for protein content, lethality, proteolytic, myotoxic, hemorrhagic, and phospholipase A2 activity. The minimum coagulant dose in plasma and human fibrinogen, protrhombin, and Factor II activations were analyzed. The venoms were also analyzed by polyacrylamide gel electrophoresis (PAGE). This showed that despite similar total protein content, the biological effects of the venoms were different. Venom from young snakes exhibited higher enzymatic and coagulant activities and higher myotoxicity compared to the mother’s. In addition, the PLA2 content paralleled myotoxicity. However, no difference could be detected in their toxicity (LD50 0.08 mg/Kg). High incidence of blood coagulation disorders and elevated circulating myoglobin may characterize systemic envenoming by young C. d. terrificus.
Documentos Relacionados
- Blood biochemical profile of the South American rattlesnake (Crotalus durissus terrificus) in captivity
- Random amplified polymorphisms between two South American subspecies of rattlesnakes (Crotalus durissus collilineatus and Crotalus durissus terrificus)
- Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats
- Daily and seasonal activity patterns of free range South-American rattlesnake (Crotalus durissus)
- ISOLATION OF AN OPHIDIAN PARAMYXOVIRUS (OPMV) IN A CAPTIVE RATTLESNAKE (Crotalus durissus terrificus) FROM BOTUCATU, SÃO PAULO STATE, BRAZIL