Ação do N-acetilmuramil-L-alanil-D-isoglutamina (MDP) na regeneração nervosa periférica. Estudo experimental em camundongos / N-acetylmuramyl-L-alanyl-D-isoglutamine on peripheral nerve regeneration. Experimental study in mice

AUTOR(ES)

Ana Paula Inoe Tomazini

DATA DE PUBLICAÇÃO

2003

RESUMO

N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) was evaluated for its effect on the regenerating peripheral neurons in adult animals. The left sciatic nerve of nine male adult mice C57BL/6J was cut, the proximal and distal nerve stumps were inserted into a polyethylene tube (PT) with 0,76 mm inner diameter, leaving a four mm distance gap. Animals were randomly distributed into two groups and received 2µl of purified preparation of collagen (Vitrogen® 2,4 mg/ml) (COL) or collagen and MDP made up in 1:1 volume ratio, obtaining a 1 mM (COL/MDP) concentration. Other four non-operated animals served as controls (NOR). After four weeks, PT with the regenerated nerve cables (RC), were processed for total myelinated axon counts and myelinated fiber diameter. The results revealed significant difference (p<0.05) in the axons count among the groups NOR (4355 ± 32), COL (1869 ± 289) and COL/MDP (2430 ± 223). There was a significant reduction in the diameter of myelinated fibers in the operated groups (COL = 3,38 µm ± 1,16 and COL/MDP = 3,54 µm ± 1,16) when compared to non-operated animals (6,19 µm ± 2,45). The L5 dorsal root ganglion (DRG) was also removed form the same mice and serially sectioned for sensory neurons count and measurement. No difference was found in the number of DRG neurons among the experimental groups (COL = 564 ± 51 and COL/MDP = 514 ± 56), which presented fewer sensory neurons compared to non-operated group (NOR = 1097 ± 142). The results indicate that the locally applied MDP stimulates peripheral nerve regeneration in mice.

ASSUNTO(S)

n-acetylmuramyl-lalanyl- d-isoglutamine nervo periférico regeneração regeneration n-acetilmuramil- l-alanil-d-isoglutamina tubulização camundongo tubulization mouse peripheral nerve

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