Ação da irradiação por laser de baixa energia HeNe nas alterações musculares causadas pela inoculação de veneno bruto de Bothrops jararacussu : estudo in vivo em ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Antivenom therapy is ineffective in neutralizing the severe local tissue damage following a snakebite envenoming. The need for an effective first-aid regimen aimed at minimizing these effects is of uttermost importance. Here we describe some effects of HeNe laser irradiation on muscle injected with B. jararacussu (jararacuçu) crude venom. The tibialis anterior muscle of adult anesthetized Wistar rats were injected in its medium third either with venom (60 ?g/0.02 ml) or saline. Three He-Ne laser incident energy densities (?. = 632.8 nm, 3 mW): 3.5 J/cm2 (single exposure); 3.5 J/cm2 (three exposures at each 20 min) and 10.5 J/cm2 were administered after 60 min post-venom and a myographic record was taken from 60 to 120 min and compared to unirradiated muscles. Serum CK levels measurements and histopathological/morphometrical analysis were also done. The energy density of 3.5 J/cm2 (single or three exposures) induced a significant decrease of 64% and 40.5%, respectively, of the injured area (15.9 ? 1.5% and 26.3 :t 4.5%, respectively, against 44.2 :t 5.7% in unirradiated ones, p<0.05), and a significant (62%) decrease of the neuromuscular blockade (3.5 J/cm2, single dose) (11.5 ? 2.5%, p<0.05) compared to the unirradiated group (28,6 ? 4.7%). Serum CK levels ranged from 455 ? 4.5% in unirradiated envenomed rats to 190.3 ? 23.4% in irradiated rats (3.5 J/cm2, single exposure) (p<0.05). We suggest that HeNe laser irradiation at appropriate dosage, reduces myonecrosis caused by jararacuçu snake venom and improves muscle performance

ASSUNTO(S)

eletromagnetismo laser

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