Ação da indometacina sobre o conteudo lipidico das celulas deciduais de camundongos : estudo histoquimico e morfometrico

AUTOR(ES)

Julio de Mello Neto

DATA DE PUBLICAÇÃO

1998

RESUMO

ln the aim to analyze the commitment of lipid droplets and their phopholipds contents in the decidual cells as prostaglandin precursors, it was loculated intraperitoneal " 40 AL of 25mg indomethacin, a powerful inhibitor of prostaglandin-synthase, solubilized in sesame oil, in the female mice on the fifth days of pregnancy. Controls animais received the same volume of sesame oil. Ali animais were sacrificed on the 7° dop. One group of embryo implantation sites. was collected and fixed in 3% HgCl2-formalin solution. Frozen sections (14lAm thick) were processed for phospholipids containing choline and Sudan Black histochemistry . Another group of lndomethacin treated and untreated animais were fixed with glutaraldehyde under - microwave treatment, postfixed, in OS04 (1%) and processed for usual epoxy resin embedding. The morphometric analysis showed the volume of lndomethacin treated uterus smaller than untreated one. At microscopic levei the decidual reaction was less proeminent in the endometrium of treated animal. The P hosp holi pid-contain in g-choli ne and Sudan Black histochemistry showed strong reaction in fully differentiated decidual cel of and less intense in the predecidual zone. When compared the intensity between untreated and treated animais, the later showed strongest reactions. The ultrastructural analyses showed the higher amount of lipid droplets inside decidual cel of Indomethacin treated than untreated animais. These findings suggest the effective action of Indomethacin on decidual reaction and affected the lipid metabolism. The high storage rates of lipids in the decidual cel and predecidual cells strongly suggests that the phospholipid contents should be the precursor of PGs. Furthermore, the inhibition of PG biosynthesis affects the progression of cells already committed to decidualization

ASSUNTO(S)

camundongo histoquimica

Documentos Relacionados

• Efeito da indometacina e da dexametasona sobre os lipidios contidos nas celulas deciduais de camundongos : estudo histoquimico e morfometrico
• Efeito da iluminação constante sobre a placenta de ratas: um estudo morfológico, morfométrico e histoquímico
• Influencia da pinealectomia sobre o cortical da adrenal : estudo morfologico e histoquimico
• Estudo histologico, histoquimico e morfometrico comparado da pele, das glandulas cutaneas e das glandulas cutaneas sexualmente dimorficas de tres especies de anfibios anuros
• Estudo histologico, histoquimico, morfometrico e ultra-estrutural da pele de duas especies de peixes teleosteos