A Ubiquitin-Conjugating Enzyme, Rad6, Affects the Distribution of Ty1 Retrotransposon Integration Positions
AUTOR(ES)
Liebman, S. W.
RESUMO
A galactose-inducible Ty1 element was used to generate 59 independent Ty1 inserts that inactivate the CAN1 gene. As found in previous studies, the distribution of these elements shows a gradient of insertion frequency from highest to lowest between the 5' and 3' ends of the gene. However, 53 independent Ty1 and Ty2 insertions isolated by an identical procedure in an isogenic rad6 deletion strain do not show this bias. In this strain, the Ty elements insert randomly throughout CAN1. These results show that the ubiquitin-conjugating enzyme, RAD6, alters the integration site preferences of Ty1 retrotransposons.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1205338Documentos Relacionados
- Mutations in RAD6, a yeast gene encoding a ubiquitin-conjugating enzyme, stimulate retrotransposition.
- The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.
- The ubiquitin-conjugating enzyme Rad6 (Ubc2) is required for silencing in Saccharomyces cerevisiae.
- Creation of a Pluripotent Ubiquitin-Conjugating Enzyme
- Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2