A STRATEGY FOR THE RAPID IDENTIFICATION OF FUNGAL METABOLITES AND THE DISCOVERY OF THE ANTIVIRAL ACTIVITY OF PYRENOCINE A AND HARZIANOPYRIDONE

Ióca, Laura P., Romminger, Stelamar, Santos, Mario F. C., Bandeira, Karin F., Rodrigues, Fabiana T., Kossuga, Miriam H., Nicacio, Karen J., Ferreira, Everton L. F., Morais-Urano, Raquel P., Passos, Messias S., Kohn, Luciana K., Arns, Clarice W., Sette, Lara D., Berlinck, Roberto G. S.

A STRATEGY FOR THE RAPID IDENTIFICATION OF FUNGAL METABOLITES AND THE DISCOVERY OF THE ANTIVIRAL ACTIVITY OF PYRENOCINE A AND HARZIANOPYRIDONE

AUTOR(ES)

Ióca, Laura P., Romminger, Stelamar, Santos, Mario F. C., Bandeira, Karin F., Rodrigues, Fabiana T., Kossuga, Miriam H., Nicacio, Karen J., Ferreira, Everton L. F., Morais-Urano, Raquel P., Passos, Messias S., Kohn, Luciana K., Arns, Clarice W., Sette, Lara D., Berlinck, Roberto G. S.

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2016-07

RESUMO

The isolation and identification of bioactive metabolites from complex extracts obtained from microbial growth media is a time consuming, costly, and labor-intensive task. A strategy to rapidly identify secondary metabolites isolated from extracts obtained from the culture media of marine-derived and endophytic fungal strains is described. Identification was achieved by HPLC-UV-MS and 1H NMR analyses in combination with data obtained from the Dictionary of Natural Products. Among the compounds identified, (-)-naphthoquinoneimine, citreorosein, emodin, pyrenocine A and harzianopyridone displayed moderate to potent antiviral activity. (-)-Naphthoquinoneimine was isolated as the enantiomer of its previously reported dextrorotatory congener, while 6,7-dihydroxy-2,2-dimethyl-4-chromanone is herein reported for the first time as a natural product.