A stable complex between homopyrimidine oligomers and the homologous regions of duplex DNAs.
AUTOR(ES)
Lyamichev, V I
RESUMO
When plasmid DNA duplexes carrying the regular homopurine-homopyrimidine inserts (dGA)n, (dTC)n and (dG)n, (dC)n are preincubated with homologous labeled oligo(dPy) ((dTC)n and (dC)n respectively) at acid pH, the label co-electrophoreses with the duplex DNA. Thus, a very strong complex is formed. Complementary oligo(dPu) does not form a complex under these conditions. No binding is observed for oligo(dPy) with non-homologous inserts as well as with vector plasmids without inserts. The complex is formed equally well with linear, nicked or superhelical DNA. The complex is not detected at pH greater than 6. Complex formation leads to very little, if any, unwinding of the duplex. The observed complex appears to be the Py.Pu.Py triplex consisting of TAT and CGC base-triads with protonated cytosines. Two-dimensional gel electrophoresis patterns show that the presence of homologous oligo(dPy) destabilizes the formation of the H form.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338206Documentos Relacionados
- Homologous recombination between transfected DNAs.
- Can recA protein promote homologous pairing between duplex regions of DNA?
- Multiple regions within EBNA1 can link DNAs.
- E. coli recA protein possesses a strand separating activity on short duplex DNAs.
- Different activities of viral enhancer elements before and after stable integration of transfected DNAs.
