A single base-pair change creates a Chi recombinational hotspot in bacteriophage lambda.
AUTOR(ES)
Sprague, K U
RESUMO
X4+ mutations, responsible for the Chi phenotype in phage lambda, locally increase the rate of recombination promoted by the Escherichia coli recombination system (Rec). X+ mutations in the cII gene, one of a few sites in lambda at which such mutations arise, were located genetically and physically with overlapping deletions. DNA sequence analysis of the deletion segment containing the X+ C mutations showed that two independent X+ C mutations arose by the same A-T to T-A transversion. Presumably, this change creates a nucleotide sequence recognized by a protein involved in a rate-limiting step of recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393143Documentos Relacionados
- Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda.
- Repair of defined single base-pair mismatches in Escherichia coli
- 1H NMR determination of base-pair lifetimes in oligonucleotides containing single base mismatches
- A structural snapshot of base-pair opening in DNA
- Photochemical selectivity in guanine–cytosine base-pair structures