A Sequence Downstream of the Initiation Codon Is Essential for Cold Shock Induction of cspB of Escherichia coli

AUTOR(ES)

Etchegaray, Jean-Pierre

FONTE

American Society for Microbiology

RESUMO

Cold shock induction of cspB has been shown to be primarily regulated at the mRNA level. Here, we demonstrate that the induction of cspB at low temperature also requires the translational cis-acting element called the downstream box (DB). Full induction of cspB at low temperature is achieved in the presence of both the Shine-Dalgarno sequence and DB. We propose that the DB sequence functions as a translational enhancer for the biosynthesis of CspB to bypass the inhibitory effect in translation caused by cold shock.

Documentos Relacionados

• Characterization of cspB, a cold-shock-inducible gene from Lactococcus lactis, and evidence for a family of genes homologous to the Escherichia coli cspA major cold shock gene.
• CspA, CspB, and CspG, Major Cold Shock Proteins of Escherichia coli, Are Induced at Low Temperature under Conditions That Completely Block Protein Synthesis
• CspI, the Ninth Member of the CspA Family of Escherichia coli, Is Induced upon Cold Shock
• Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures.
• Crystal structure of CspA, the major cold shock protein of Escherichia coli.