A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study
AUTOR(ES)
Bedor, Danilo Cesar Galindo, Souza Filho, Jose Homero de, Ramos, Virna Ligiane Souza, Gonçalves, Talita Mota, Sousa, Carlos Eduardo Miranda de, Santana, Davi Pereira de
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2011
RESUMO
An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 - 5000 ng mL-1. The mean recovery was 83%. Intra- and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study.
Documentos Relacionados
- Quick and simple LC-MS/MS method for the determination of simvastatin in human plasma: application to pharmacokinetics and bioequivalence studies
- A SIMPLE AND SENSITIVE LC-MS/MS METHOD FOR THE DETERMINATION OF S-PHENYLMERCAPTURIC ACID IN HUMAN URINE
- Simultaneous determination of candesartan and hydrochlorothiazide in human plasma by LC-MS/MS
- Simultaneous Quantification of Plasma Catecholamines and Metanephrines by LC-MS/MS
- GC–MS and LC–MS/MS workflows for the identification and quantitation of pyrrolizidine alkaloids in plant extracts, a case study: Echium plantagineum